Composite

Part:BBa_K4166014:Design

Designed by: Alexa DeKorte, Christina Harbin   Group: iGEM22_Alma   (2022-10-11)


This is a control for our kill switch which is activated at low AHL concentrations


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 918
    Illegal NheI site found at 941
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1612
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 331
    Illegal AgeI site found at 3035
    Illegal AgeI site found at 3147
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The fragments ordered were split up in size for convenience and to avoid problems with IDT synthesis



Source

DNA ordered from IDT

References