Composite
Part:BBa_K4166014:Design
Designed by: Alexa DeKorte, Christina Harbin Group: iGEM22_Alma (2022-10-11)
This is a control for our kill switch which is activated at low AHL concentrations
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 918
Illegal NheI site found at 941 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1612
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 331
Illegal AgeI site found at 3035
Illegal AgeI site found at 3147 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The fragments ordered were split up in size for convenience and to avoid problems with IDT synthesis
Source
DNA ordered from IDT